ACHEMS 2019
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SPLTRAK Abstract Submission
Target Specific Functions of EPL Interneurons in Olfactory Circuits
Gary Liu2,4,5, Emmanoil Froudarakis3, Jay Patel3,4, Mikhail Kochukov1, Brandon Pekarek1, Patrick Hunt1,4, Mayuri Patel2, Kevin Ung2, Annie Fu3, Juyeong Jo3, Hyun-Kyoung Lee3,5, Andreas S. Tolias3, Benjamin R. Arenkiel1,2,3,5
1Department of Molecular & Human Genetics, Baylor College of Medicine, Houston, TX, United States
2Program in Developmental Biology, Baylor College of Medicine, Houston, TX, United States
3Department of Neuroscience, Baylor College of Medicine, Houston, TX, United States
4Medical Scientist Training Program, Baylor College of Medicine, Houston, TX, United States
5Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital, Houston, TX, United States

Inhibitory interneurons play prominent roles in sensory processing, yet their target-specific functions in sensory circuits remain largely unknown. Investigating the mouse olfactory system, we selectively removed GABA transmission from a subset of olfactory bulb interneurons, and assayed odor responses from their downstream synaptic partners — Tufted cells and Mitral cells. Using a combination of in vivo electrophysiological and imaging analyses, we found that inactivating this single node of inhibition leads to cellular changes in Mitral/Tufted cell odor responsivity, with differential effects in intensity, reliability, tuning, temporal dynamics and mixture coding between the two principal neuron cell types. Together, our data suggest that olfactory bulb interneurons, through exerting distinct inhibitory functions onto their different synaptic partners, can increase the dimensionality of sensory processing.