SPLTRAK Abstract Submission
The neuroplastic effect of olfactory training to the recovery of olfactory system in mouse model
Boo-Young Kim1 & Sang-uk Lee2
1Catholic university, Otorhinolaryngology department, Uijeoungbu, Korea
2catholic university, Orthopedics, Incheon, Korea

Objective: Several studies have reported the benefits of olfactory training (OT) in the olfactory nervous system of mouse models. Therefore, next-generation sequencing was performed to evaluate the effects of OT on mRNA sequencing in the olfactory area. Subjects and Methods: Mice in each group were administered 300 mg of 3-methylindole per kg of mouse weight. The olfactory function was evaluated by a food-finding test once a week. The olfactory neuroepithelium was harvested for histologic examination and protein analysis. Subsequently, data analysis, gene ontology and pathway analysis, quantitative real-time polymerase chain reaction of mRNA, and western blot analysis were conducted. Results: Mice were divided into four groups according to treatment. Control, anosmia, training, and steroid group mice resumed food finding. Olfactory Maker Protein, olfr1507, ADCY3, and GNAL mRNA expression was higher in the olfactory neuroepithelium of OT than anosmia group mice. In total, 26,364 mRNAs were differentially expressed. Comparison of the results of OT versus anosmia revealed that ADCY8,10, GFAP, NGF, NGFR, GFAP, and BDNF mRNAs were upregulated in the gene ontology. Conclusions: OT improved olfactory function, as indicated by the food finding test. OT improved the olfactory recovery time to stimulate olfactory nerve regeneration. OT might initially stimulate the olfactory receptor, followed by neurogenesis. Steroid therapy and OT operate under completely different mechanisms in the upregulated gene study. These results indicate that OT might be one of the future modalities for treating olfactory impairment.