SPLTRAK Abstract Submission
Fructose Signal Transduction in Cultured Human Fungiform Taste Papillae (HBO) Cells 
M. Hakan Ozdener, Evangeline Adjei-Danquah, Robert F. Margolskee
Monell Chemical Senses Center, Philadelphia, PA, United States

Fructose and fructose derived products are a significant component of the human diet and have been related to diabetes as well as insulin resistance. Although there are many reports on how fructose is absorbed and transported in the intestine, it is not known if such transporters in human taste cells might contribute to how we detect fructose. We examined which receptors, transporters and metabolic pathways might play a role in fructose detection by taste cells using cultured human fungiform taste (HBO) cells. Using Ca++ indicator or the voltage sensitive membrane dyes, we found that HBO cells responded in a dose-dependent manner to increasing concentrations of fructose. Blocking TAS1R3 with lactisole and knocking down glucose transporter 5 (Glut5), known to transport fructose, by siRNA significantly decreased responses of HBO cells to fructose, indicating that human taste cells detect fructose by TAS1R3 and Glut5. Pharmacological modulators of the KATP channels did not alter HBO cell responses to fructose indicating that they are not involved. In conclusion, human taste cells respond to fructose via TAS1R3 and Glut5 but not KATP.