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SPLTRAK Abstract Submission
Single Cell Transcriptome Analysis of Olfactory Sensory Neurons
Delia Tomoiaga3, Raena Mina1, 2, Eugene Lempert1, 2, Christopher E Mason3, 4, Paul Feinstein1, 2
1Hunter College, CUNY, Department of Biological Sciences, New York, NY, United States
2Graduate Center of CUNY, Biochemistry, MCD, and CCP Programs , New York, NY, United States
3Weill Cornell Medicine, Department of Physiology and Biophysics, New York, NY, United States
4Weill Cornell Medicine, Tri-institutional Training Program in Computational Biology and Medicine, New York, NY, United States

  The olfactory sensory neurons of the olfactory system in mammals detects odors by an enormous superfamily of G-protein coupled receptor genes, aptly named as odorant receptors (ORs) genes.  This gene family number 1500 genes in the mouse and about 1000 in humans, but not all of the ORs produce functional proteins. In the mouse, the functional repertoire is believed to number around 1200 genes. The lack of selective pressure on these genes provides for the possibility that each allele of each gene encodes a distinct protein. Thus, the repertoire of functional OR alleles could be as high as 2400. The biology of the olfactory system is set up in such a way that the expression of one functional OR allele inhibits the expression of any other OR allele.  This functional OR allele also provides the axons of their neurons the ability to project homotypically into homogeneous glomeruli in the olfactory bulb. As such, each of the 2400 OR alleles, represented by approximately ~4000 neurons on average, send projections to the olfactory bulb.  A few years ago, we published the MouSensor technology1, which increases the representation of a given cloned OR allele such that it is represented by 500,000 or more neurons. We are using this technology to characterize the transcriptome of many neurons that have selected the same OR allele for expression. Our preliminary mRNA analysis suggests that MouSensor OR transgene expressing neurons only express the MouSensor OR", which is consistent with our previous axon guidance data.                                                                                  1MouSensor: A Versatile Genetic Platform to Create Super Sniffer Mice for Studying Human Odor Coding. (2016) Charlotte D’Hulst, Raena B. Mina, Zachary Gershon, Sophie Jamet, AntonioCerullo, Delia Tomoiaga, Li Bai, Leonardo Belluscio, Matthew Rogers, Yevgeniy Sirotin, and Paul Feinstein. Cell Rep. 2016 Jul 26;16(4):1115-25. doi: 10.1016/j.celrep.2016.06.047. Epub 2016 Jul 7.