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SPLTRAK Abstract Submission
Chemosensory Receptors of Cydia pomonella (Lepidoptera: Tortricidae)
Alberto Maria Cattaneo1, Yuriy V. Bobkov2, Francisco Gonzalez3, Stephen F. Garczynski4, Peter Witzgall1, William B. Walker III1
1Swedish University of Agricultural Sciences, Department of Plant Protection Biology, Chemical Ecology Horticulture Research Unit, Alnarp, Sweden
2University of Florida, Whitney Laboratory, Center for Smell and Taste, and McKnight Brain Institute, Gainesville, FL, United States
3ChemTica Internacional S.A., Sto. Domingo, Heredia, Costa Rica
4USDA-ARS, Temperate Tree Fruit and Vegetable Research Unit, Wapato, WA, United States

Cydia pomonella (Lepidoptera: Tortricidae) is a major pest of apple, pear and walnuts. For its control, alternative strategies targeting the olfactory system, such as mating disruption, have been combined with insecticide applications. The efficacy of these strategies guided the direction of efforts for the functional characterization of codling moth chemosensory receptors to implement further control methods based on targeting chemical sensing. With the advent of transcriptomic analysis, chemosensory receptors transcripts have been identified in antennal transcriptomes of C. pomonella. Heterologous expression of full-length chemosensory receptor open reading frames in empty neurons of Drosophila melanogaster and in Human Embryonic Kidney cells allowed detailed functional studies of receptors including ligand specificity (deorphanization) and receptors' activity patterns. Among different classes of antennal receptors, several odorant receptors of C. pomonella (CpomORs) have been characterized as specific for kairomones (CpomOR3), pheromones (CpomOR6a) and compounds emitted by non-host plants (CpomOR19). Physiological and pharmacological studies of these receptors confirmed their ionotropic properties, by forming functional channels with the co-receptor subunit of CpomOrco. Interestingly, molecular biological analysis suggests that at least some receptors of C. pomonella are expressed as a complex combination of splice-isoforms and their expression pattern may correlate with the expression of other ion channels (e.g. CpomOR53 and TRPA5). Investigation of chemosensory capabilities in the codling moth has practical outcomes for the development of control strategies and has inspired novel trends to control this pest by integrating alternative methods to interfere with insect chemosensory communication.